Practical Guide: Peptide Reconstitution and Storage

Lyophilized peptides are stable during transport at room temperature, but their long-term storage requires specific precautions.

BEFORE RECONSTITUTION

• Optimal storage: -20°C (standard freezer)
• Shelf life: up to 24 months
• Absolutely avoid repeated freeze/thaw cycles
• Protect from moisture and direct light

RECONSTITUTION For most water-soluble peptides:

• Use bacteriostatic water (0.9% benzyl alcohol)
• Add the solvent gently along the vial wall
• Let dissolve naturally - do not shake vigorously
• Wait 5-10 minutes for the solution to be homogeneous

For hydrophobic peptides (containing lots of Leu, Ile, Val, Met, Phe):

• Pre-dissolve in 10% acetic acid or DMSO
• Then dilute with bacteriostatic water

AFTER RECONSTITUTION

• Storage at 4°C: 2 to 4 weeks maximum
• Storage at -20°C in aliquots: several months
• Always use sterile equipment
• Note the reconstitution date on the vial

A degraded peptide gradually loses its effectiveness without visible signs. When in doubt, a new batch is preferable.